ArtPoster
TitelHypoxic-ischaemic encephalopathy – microRNA expression profiles as a prognostic marker for neurological outcome
TopicNeonatologie
Einleitung

Hypoxic-ischaemic encephalopathy (HIE) following perinatal asphyxia is a major cause of brain injury and neurodevelopmental delay in newborn infants. Prognosis is based on clinical, neuro-imaging and neurophysiological findings. To ensure higher accuracy, new approaches are called for. Previously, we revealed alterations in micro ribonucleic acids (miRNA) expression profiles of asphyxiated neonates compared to healthy controls. The aim of the current study was to assess miRNA expression profiles as a potential prognostic biomarker for neurological outcome of asphyxiated infants suffering from HIE.

Patient/en und Methoden

This study is a retrospective analysis of prospectively enrolled subjects admitted to NICU Innsbruck between January 2017 and December 2018. The patient group included asphyxiated newborn infants with a gestational age of ≥36 weeks fulfilling the diagnostic criteria of moderate/severe HIE according to Sarnat & Sarnat. Subjects routinely underwent neurological follow-up by means of neuropaediatric examination, general movement assessment and Bayley Scales of Infant Development III® during their first year of life. Healthy full-term infants served as controls. 48h after birth, dried blood spots were collected from venous blood. Samples were analysed by means of the FirePlexTM particle technology for multiplex miRNA assay (Abcam FirePlex Service Lab, Cambridge, USA).

Ergebnisse

MiRNA expression profiles were analysed from 42 neonates from the following groups: healthy controls (n=34), asphyxiated infants suffering from moderate/severe HIE with good neurological outcome (n=6), and asphyxiated infants suffering from moderate/severe HIE with poor neurological outcome (n=2). Infants with poor neurological outcome showed a significant decrease of hsa-mir-125b-5p and hsa-mir-16-2-3p, and a significant increase of hsa-mir-24-3p and hsa-mir-532-5p in comparison to healthy controls. Furthermore, they showed a significant increase of hsa-mir-145-5p compared to infants suffering from moderate/severe HIE with good neurological outcome (Mann-Whitney U test, significance Bonferroni-corrected: all p < 0.05).

Schlussfolgerung/Diskussion

Infants with poor neurological outcome following HIE show alterations in miRNA expression profiles in comparison to case infants with good neurological outcome and healthy controls. Hence, miRNA expression profiling is a promising novel approach for predicting, within a few days of birth, the neurodevelopmental outcome of infants suffering from HIE and may facilitate clinical management and counselling of parents. Additional studies are required.

review
Erstautor*in ist unter 35 Jahre alt
Autor*in 1Ira Winkler Department of Pediatrics II, Medical University of Innsbruck, Innsbruck, Austria
Autor*in 2Tatjana Heisinger Department of Pediatrics II, Medical University of Innsbruck, Innsbruck, Austria
Autor*in 3Eva Huber Department of Pediatrics II, Medical University of Innsbruck, Innsbruck, Austria
Autor*in 4Martina Urbanek Department of Pediatrics II, Medical University of Innsbruck, Innsbruck, Austria
Autor*in 5Marlene Hammerl Department of Pediatrics II, Medical University of Innsbruck, Innsbruck, Austria
Autor*in 6Ursula Kiechl-Kohlendorfer Department of Pediatrics II, Medical University of Innsbruck, Innsbruck, Austria
Autor*in 7Elke Griesmaier Department of Pediatrics II, Medical University of Innsbruck, Innsbruck, Austria
Autor*in 8Anna Posod Department of Pediatrics II, Medical University of Innsbruck, Innsbruck, Austria